Recombinant Human MED25 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MED25-848H
• Product Overview: MED25 MS Standard C13 and N15-labeled recombinant protein (NP_112235) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a component of the transcriptional coactivator complex termed the Mediator complex. This complex is required for transcription of most RNA polymerase II-dependent genes. The encoded protein plays a role in chromatin modification and in preinitiation complex assembly. Mutations in this gene are associated with Charcot-Marie-Tooth disease type 2B2.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 78.2 kDa
• AA Sequence: MVPGSEGPARAGSVVADVVFVIEGT ANLGPYFEGLRKHYLLPAIEYFNGG PPAETDFGGDYGGTQYSLVVFNTVD CAPESYVQCHAPTSSAYEFVTWLDG IKFMGGGGESCSLIAEGLSTALQLF DDFKKMREQIGQTHRVCLLICNSPP YLLPAVESTTYSGCTTENLVQQIGE RGIHFSIVSPRKLPALRLLFEKAAP PALLEPLQPPTDVSQDPRHMVLVRG LVLPVGGGSAPGPLQSKQPVPLPPA APSGATLSAAPQQPLPPVPPQYQVP GNLSAAQVAAQNAVEAAKNQKAGLG PRFSPITPLQQAAPGVGPPFSQAPA PQLPPGPPGAPKPPPASQPSLVSTV APGSGLAPTAQPGAPSMAGTVAPGG VSGPSPAQLGAPALGGQQSVSNKLL AWSGVLEWQEKPKPASVDANTKLTR SLPCQVYVNHGENLKTEQWPQKLIM QLIPQQLLTTLGPLFRNSRMVQFHF TNKDLESLKGLYRIMGNGFAGCVHF PHTAPCEVRVLMLLYSSKKKIFMGL IPYDQSGFVNGIRQVITNHKQVQQQ KLEQQQRGMGGQQAPPGLGPILEDQ ARPSQNLLQLRPPQPQPQGTVGASG ATGQPQPQGTAQPPPGAPQGPPGAA SGPPPPGPILRPQNPGANPQLRSLL LNPPPPQTGVPPPQASLHHLQPPGA PALLPPPHQGLGQPQLGPPLLHPPP AQSWPAQLPPRAPLPGQMLLSGGPR GPVPQPGLQPSVMEDDILMDLITRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MED25 mediator complex subunit 25 [ Homo sapiens (human) ]
• Official Symbol: MED25
• Synonyms: MED25; mediator complex subunit 25; mediator of RNA polymerase II transcription, subunit 25 homolog (S. cerevisiae); mediator of RNA polymerase II transcription subunit 25; ACID1; ARC92; DKFZp434K0512; TCBAP0758; prostate-derived protein 78 kDa; activator-recruited cofactor 92 kDa component; ARC/mediator transcriptional coactivator subunit; activator interaction domain-containing protein 1; mediator of RNA polymerase II transcription, subunit 25 homolog; P78; PTOV2; CMT2B2; MGC70671
• Gene ID: 81857
• mRNA Refseq: NM_030973
• Protein Refseq: NP_112235
• MIM: 610197
• UniProt ID: Q71SY5