Recombinant Human METTL3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: METTL3-1630H
• Product Overview: METTL3 MS Standard C13 and N15-labeled recombinant protein (NP_062826) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes the 70 kDa subunit of MT-A which is part of N6-adenosine-methyltransferase. This enzyme is involved in the posttranscriptional methylation of internal adenosine residues in eukaryotic mRNAs, forming N6-methyladenosine.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 64.3 kDa
• AA Sequence: MSDTWSSIQAHKKQLDSLRERLQRR RKQDSGHLDLRNPEAALSPTFRSDS PVPTAPTSGGPKPSTASAVPELATD PELEKKLLHHLSDLALTLPTDAVSI CLAISTPDAPATQDGVESLLQKFAA QELIEVKRGLLQDDAHPTLVTYADH SKLSAMMGAVAEKKGPGEVAGTVTG QKRRAEQDSTTVAAFASSLVSGLNS SASEPAKEPAKKSRKHAASDVDLEI ESLLNQQSTKEQQSKKVSQEILELL NTTTAKEQSIVEKFRSRGRAQVQEF CDYGTKEECMKASDADRPCRKLHFR RIINKHTDESLGDCSFLNTCFHMDT CKYVHYEIDACMDSEAPGSKDHTPS QELALTQSVGGDSSADRLFPPQWIC CDIRYLDVSILGKFAVVMADPPWDI HMELPYGTLTDDEMRRLNIPVLQDD GFLFLWVTGRAMELGRECLNLWGYE RVDEIIWVKTNQLQRIIRTGRTGHW LNHGKEHCLVGVKGNPQGFNQGLDC DVIVAEVRSTSHKPDEIYGMIERLS PGTRKIELFGRPHNVQPNWITLGNQ LDGIHLLDPDVVARFKQRYPDGIIS KPKNLTRTRPLEQKLISEEDLAAND ILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: METTL3 methyltransferase like 3 [ Homo sapiens (human) ]
• Official Symbol: METTL3
• Synonyms: METTL3; methyltransferase like 3; M6A; IME4; Spo8; MT-A70; hMETTL3; N6-adenosine-methyltransferase catalytic subunit; N6-adenosine-methyltransferase 70 kDa subunit; adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase; mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase; mRNA m(6)A methyltransferase; methyltransferase-like protein 3; EC 2.1.1.348
• Gene ID: 56339
• mRNA Refseq: NM_019852
• Protein Refseq: NP_062826
• MIM: 612472
• UniProt ID: Q86U44