Active Recombinant Human MSLN Protein, Fc-tagged

• Cat.No.: MSLN-10625H
• Product Overview: Recombinant human MSLN-Fc fusion protein is expressed as a 534-amino acid protein consisting of Glu296 - Ser592 region of MSLN (UniProt accession #Q13421, isoform 2) and a C-terminal Fc from human IgG1, which exists as a dimer under non-reducing conditions.

Specification

• Source: Human cells
• Species: Human
• Tag: Fc
• Bio-activity: Binds to CA125/MUC16 and anti-MSLN monoclonal antibody, human IgG1 with high affinity. Blocks MSLN's binding to CA125/MUC16.
• Molecular Mass: Calculated molecular mass (kDa): 60.1; Estimated by SDS-PAGE under reducing condition (kDa): 65-70
• AA Sequence: EVEKTACPSGKKAREIDESLIFYKK WELEACVDAALLATQMDRVNAIPFT YEQLDVLKHKLDELYPQGYPESVIQ HLGYLFLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLIDRFVK GRGQLDKDTLDTLTAFYPGYLCSLS PEELSSVPPSSIWAVRPQDLDTCDP RQLDVLYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQNVSMDL ATFMKLRTDAVLPLTVAEVQKLLGP HVEGLKAEERHRPVRDWILRQRQDD LDTLGLGLQGGIPNGYLVLDLSTTE NLYFQGSTGTHTCPPCPAPELLGGP SVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPEVKFNWYVDGVEVHNAK TKPREEQYNSTYRVVSVLTVLHQDW LNGKEYKCKVSNKALPAPIEKTISK AKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPE NNYKTTPPVLDSDGSFFLYSKLTVD KSRWQQGNVFSCSVMHEALHNHYTQ KSLSLSPGK
• Endotoxin: <0.1 EU per 1 μg of purified recombinant protein determined by the LAL method
• Purity: >95% judged by SDS-PAGE under reducing condition
• Storage: The product is shipped at 4 centigrade. Upon receipt, centrifuge the product briefly before opening the vial. It is recommended to store small aliquots at the temperature below –20 centigrade for long-term storage and the product is stable for 3 months. The undiluted protein can be stored at 4 centigrade for no more than 2 weeks.
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). The purified recombinant protein was labeled with Biotin (3-5 Biotin per molecule) using the standard procedure.

Gene Information

• Gene Name: MSLN
• Official Symbol: MSLN mesothelin [ Homo sapiens (human) ]
• Synonyms: Mesothelin; MPF; SMRP; MSLN; mesothelin; CAK1 antigen; megakaryocyte potentiating factor; pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
• Gene ID: 10232
• mRNA Refseq: NM_005823
• Protein Refseq: NP_005814
• MIM: 601051
• UniProt ID: Q13421