Recombinant Human NDUFV1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: NDUFV1-2770H
• Product Overview: NDUFV1 MS Standard C13 and N15-labeled recombinant protein (NP_009034) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The mitochondrial respiratory chain provides energy to cells via oxidative phosphorylation and consists of four membrane-bound electron-transporting protein complexes (I-IV) and an ATP synthase (complex V). This gene encodes a 51 kDa subunit of the NADH:ubiquinone oxidoreductase complex I; a large complex with at least 45 nuclear and mitochondrial encoded subunits that liberates electrons from NADH and channels them to ubiquinone. This subunit carries the NADH-binding site as well as flavin mononucleotide (FMN)- and Fe-S-biding sites. Defects in complex I are a common cause of mitochondrial dysfunction; a syndrome that occurs in approximately 1 in 10,000 live births. Mitochondrial complex I deficiency is linked to myopathies, encephalomyopathies, and neurodegenerative disorders such as Parkinson's disease and Leigh syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 50.8 kDa
• AA Sequence: MLATRRLLGWSLPARVSVRFSGDTT APKKTSFGSLKDEDRIFTNLYGRHD WRLKGSLSRGDWYKTKEILLKGPDW ILGEIKTSGLRGRGGAGFPTGLKWS FMNKPSDGRPKYLVVNADEGEPGTC KDREILRHDPHKLLEGCLVGGRAMG ARAAYIYIRGEFYNEASNLQVAIRE AYEAGLIGKNACGSGYDFDVFVVRG AGAYICGEETALIESIEGKQGKPRL KPPFPADVGVFGCPTTVANVETVAV SPTICRRGGTWFAGFGRERNSGTKL FNISGHVNHPCTVEEEMSVPLKELI EKHAGGVTGGWDNLLAVIPGGSSTP LIPKSVCETVLMDFDALVQAQTGLG TAAVIVMDRSTDIVKAIARLIEFYK HESCGQCTPCREGVDWMNKVMARFV RGDARPAEIDSLWEISKQIEGHTIC ALGDGAAWPVQGLIRHFRPELEERM QRFAQQHQARQAASTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: NDUFV1 NADH:ubiquinone oxidoreductase core subunit V1 [ Homo sapiens (human) ]
• Official Symbol: NDUFV1
• Synonyms: NDUFV1; NADH dehydrogenase (ubiquinone) flavoprotein 1, 51kDa; NADH dehydrogenase (ubiquinone) flavoprotein 1 (51kD); NADH dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial; CI 51K; complex I 51kDa subunit; NADH dehydrogenase [ubiquinone] flavoprotein 1; mitochondrial; complex I 51 kda subunit; complex I, mitochondrial respiratory chain; NADH-ubiquinone oxidoreductase 51 kDa subunit; mitochondrial NADH dehydrogenase ubiquinone flavoprotein 1; mitochondrial NADH:ubiquinone oxidoreductase 51 kda subunit; UQOR1; CI-51K; CI51KD; FLJ59059
• Gene ID: 4723
• mRNA Refseq: NM_007103
• Protein Refseq: NP_009034
• MIM: 161015
• UniProt ID: P49821