Active Recombinant Human NLGN4Y protein, His-tagged

• Cat.No.: NLGN4Y-25H
• Product Overview: Recombinant Human NLGN4Y(Gln42-Val677) fused with His tag at C-terminal was expressed in CHO.

Specification

• Source: CHO
• Species: Human
• Tag: His
• Predicted N Terminal: No results obtained. Gln42 inferred from enzymatic pyroglutamate treatment revealing Ala43
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-12 µg/mL.
• Molecular Mass: Predicted Molecular Mass: 72 kDa
  SDS-PAGE: 90-105 kDa, reducing conditions
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Stability: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: NLGN4Y neuroligin 4, Y-linked [ Homo sapiens ]
• Official Symbol: NLGN4Y
• Synonyms: NLGN4Y; neuroligin 4, Y-linked; neuroligin 4, Y linked; neuroligin-4, Y-linked; KIAA0951
• Gene ID: 22829
• mRNA Refseq: NM_001164238
• Protein Refseq: NP_001157710
• MIM: 400028
• UniProt ID: Q8NFZ3
• Chromosome Location: Y
• Function: NOT carboxylesterase activity; neurexin family protein binding; protein binding; receptor activity