| Description : |
This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
129.6 kDa |
| AA Sequence : |
MLKFRTVHGGLRLLGIRRTSTAPAA SPNVRRLEYKPIKKVMVANRGEIAI RVFRACTELGIRTVAIYSEQDTGQM HRQKADEAYLIGRGLAPVQAYLHIP DIIKVAKENNVDAVHPGYGFLSERA DFAQACQDAGVRFIGPSPEVVRKMG DKVEARAIAIAAGVPVVPGTDAPIT SLHEAHEFSNTYGFPIIFKAAYGGG GRGMRVVHSYEELEENYTRAYSEAL AAFGNGALFVEKFIEKPRHIEVQIL GDQYGNILHLYERDCSIQRRHQKVV EIAPAAHLDPQLRTRLTSDSVKLAK QVGYENAGTVEFLVDRHGKHYFIEV NSRLQVEHTVTEEITDVDLVHAQIH VAEGRSLPDLGLRQENIRINGCAIQ CRVTTEDPARSFQPDTGRIEVFRSG EGMGIRLDNASAFQGAVISPHYDSL LVKVIAHGKDHPTAATKMSRALAEF RVRGVKTNIAFLQNVLNNQQFLAGT VDTQFIDENPELFQLRPAQNRAQKL LHYLGHVMVNGPTTPIPVKASPSPT DPVVPAVPIGPPPAGFRDILLREGP EGFARAVRNHPGLLLMDTTFRDAHQ SLLATRVRTHDLKKIAPYVAHNFSK LFSMENWGGATFDVAMRFLYECPWR RLQELRELIPNIPFQMLLRGANAVG YTNYPDNVVFKFCEVAKENGMDVFR VFDSLNYLPNMLLGMEAAGSAGGVV EAAISYTGDVADPSRTKYSLQYYMG LAEELVRAGTHILCIKDMAGLLKPT ACTMLVSSLRDRFPDLPLHIHTHDT SGAGVAAMLACAQAGADVVDVAADS MSGMTSQPSMGALVACTRGTPLDTE VPMERVFDYSEYWEGARGLYAAFDC TATMKSGNSDVYENEIPGGQYTNLH FQAHSMGLGSKFKEVKKAYVEANQM LGDLIKVTPSSKIVGDLAQFMVQNG LSRAEAEAQAEELSFPRSVVEFLQG YIGVPHGGFPEPFRSKVLKDLPRVE GRPGASLPPLDLQALEKELVDRHGE EVTPEDVLSAAMYPDVFAHFKDFTA TFGPLDSLNTRLFLQGPKIAEEFEV ELERGKTLHIKALAVSDLNRAGQRQ VFFELNGQLRSILVKDTQAMKEMHF HPKALKDVKGQIGAPMPGKVIDIKV VAGAKVAKGQPLCVLSAMKMETVVT SPMEGTVRKVHVTKDMTLEGDDLIL EIETRTRPLEQKLISEEDLAANDIL DYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
