Active Recombinant Human PDCD1 Protein, Fc-tagged, Alexa Fluor® 488 conjugated

• Cat.No.: PDCD1-21H
• Product Overview: Recombinant human Alexa Fluor® 488 conjugated PD-1 (Leu25-Gln167) protein with human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in mouse myeloma cell line.

Specification

• Description: Programmed cell death protein 1 (PDCD1) is an immune-inhibitory receptor expressed in activated T cells; it is involved in the regulation of T-cell functions, including those of effector CD8+ T cells. In addition, this protein can also promote the differentiation of CD4+ T cells into T regulatory cells. PDCD1 is expressed in many types of tumors including melanomas, and has demonstrated to play a role in anti-tumor immunity. Moreover, this protein has been shown to be involved in safeguarding against autoimmunity, however, it can also contribute to the inhibition of effective anti-tumor and anti-microbial immunity.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: Fc
• Form: Disulfide-linked homodimer
  Labeled with Alexa Fluor® 488 via amines
  Excitation Wavelength: 488 nm
  Emission Wavelength: 515-545 nm
• Bio-activity: Measured by flow cytometry for its ability to bind anti-human PD-1 Monoclonal Antibody conjugated beads. The concentration of Recombinant Human PD-1 Fc Chimera Alexa Fluor® 488 that produces 50% of the binding response is 5.00-50.0 ng/mL.
• Molecular Mass: 42.6 kDa
• N-terminal Sequence Analysis: Leu25
• Endotoxin: <1.0 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  6 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after opening.
  3 months, -20 to -70 centigrade under sterile conditions after opening.
• Storage Buffer: Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped with dry ice or equivalent.

Gene Information

• Gene Name: PDCD1 programmed cell death 1 [ Homo sapiens (human) ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1; programmed cell death protein 1; programmed cell death 1 protein; protein PD-1; systemic lupus erythematosus susceptibility 2
• Gene ID: 5133
• mRNA Refseq: NM_005018
• Protein Refseq: NP_005009
• MIM: 600244
• UniProt ID: Q15116