Active Recombinant Human PDCD1 Protein, LIgG2b Fc-tagged, low endotoxin
Cat.No. : | PDCD1-361H |
Product Overview : | Recombinant Human PDCD1 Protein, LIgG2b Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1). Predicted N-terminus: Leu 25. This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
- Specification
- Gene Information
- Related Products
Source : | HEK293 |
Species : | Human |
Tag : | LIgG2b Fc |
Form : | Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization. |
Bio-activity : | Immobilized Human PD-L1, Mouse IgG1 Fc Tag (Cat. No. PD1-H52A3) at 0.5 μg/mL (100 μL/well) can bind Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) with a linear range of 20-313 ng/mL (QC tested). Immobilized Human PD-1, Llama IgG2b Fc Tag, low endotoxin (Cat. No. PD1-H5259) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL (Routinely tested). |
Molecular Mass : | 43.8 kDa |
Protein length : | Leu 25 - Gln 167 |
Endotoxin : | Less than 0.01 EU per μg by the LAL method. |
Purity : | >95% as determined by SDS-PAGE. |
Notes : | Please avoid repeated freeze-thaw cycles. |
Stability : | No activity loss is observed after storage at: 4-8 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. |
Storage : | For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents. |
Gene Name : | PDCD1 programmed cell death 1 [ Homo sapiens ] |
Official Symbol : | PDCD1 |
Synonyms : | PDCD1; programmed cell death protein 1; CD279; PD1; protein PD-1; PD-1; SLEB2; hPD-1; hPD-l; |
Gene ID : | 5133 |
mRNA Refseq : | NM_005018 |
Protein Refseq : | NP_005009 |
MIM : | 600244 |
UniProt ID : | Q15116 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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