| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
mFc |
| Form : |
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose. |
| Bio-activity : |
Immobilized Human PD-L1, Fc Tag at 10 μg/mL (100 μL/well) can bind Human PD-1, Mouse IgG2a Fc Tag with a linear range of 4-63 ng/mL. Immobilized Human PD-1, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Human PD-L2, Fc Tag with a linear range of 0.2-4 ng/mL. Immobilized Human PD-1, Mouse IgG2a Fc Tag at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-2 ng/mL. Immobilized Human PD-L1, His Tag at series of concentration on MonRabTM Anti-His Tag (C-term) Antibody precoated (0.1 μg/well) plate, can bind Human PD-1, Mouse IgG2a Fc Tag at 5 μg/mL (100 μL/well) with a linear range of 10-78 ng/mL. |
| Molecular Mass : |
This protein carries a mouse IgG2a Fc tag at the C-terminus.The protein has a calculated MW of 42.5 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
| Protein Length : |
Leu 25 - Gln 167 |
| Endotoxin : |
Less than 0.1 EU per μg by the LAL method. |
| Purity : |
>95% as determined by SDS-PAGE. >90% as determined by SEC-HPLC. |
| Storage : |
For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
