"PDGFB" Related Products

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Recombinant Human PDGFB Protein, His & Avi-tagged, Unconjugated

Cat.No.: PDGFB-377H
Product Overview: Recombinant Human PDGFB Protein, His & Avi-tagged, Unconjugated is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127). Predicted N-terminus: Ser 82. This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag. The protein has a calculated MW of 15.0 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).
Source: HEK293
Species: Human
Tag : His & Avi
Form: Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Recombinant Human PDGFB Protein, His & Avi-tagged, Unconjugated at 5 µg/mL (100 µl/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.02-1.2 µg/mL (QC tested).
Molecular Mass: 15.0 kDa
Protein length: Ser 82 - Thr 190
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: PDGFB platelet-derived growth factor beta polypeptide [ Homo sapiens ]
Official Symbol: PDGFB
Synonyms: PDGFB; becaplermin; oncogene SIS; SSV; PDGF-2; PDGF, B chain; PDGF subunit B; proto-oncogene c-Sis; SIS; PDGF2; c-sis; FLJ12858;
Gene ID: 5155
mRNA Refseq: NM_002608
Protein Refseq: NP_002599
MIM: 190040
UniProt ID: P01127

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