Active Recombinant Human PDGFRB protein, Fc/Avi-tagged, Biotinylated
Cat.No. : | PDGFRB-051H |
Product Overview : | Biotinylated Recombinant Human PDGFRB(Leu33-Lys531) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells . |
- Specification
- Gene Information
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Description : | PDGF is a major serum mitogen that can exist as a homo or hetero-dimeric protein consisting of disulfide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Where as PDGF R alpha binds all three PDGF isoforms with high affinity, PDGF R beta binds PDGF-BB only with high-affinity. Both PDGF R alpha and PDGF R beta are members of the class III subfamily of receptor tyrosine kinases (RTK) that also includes the receptors for M-CSF, SCF and Flt3 ligand. All class III RTKs are characterized by the presence of five immunoglobulin-like domains in their extracellular region and a split kinase domain in their intracellular region. PDGF binding induces receptor homo-and hetero-dimerization and signal transduction. The expression of the alpha and beta receptors is independently regulated in various cell types. Recombinant soluble PDGF R beta binds PDGF with high affinity and is potent PDGF antagonist. Our Avi-tag Biotinylated Human PDGF R beta features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity |
Source : | CHO cells |
Species : | Human |
Tag : | Fc/Avi |
Predicted N Terminal : | Leu33 |
Form : | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Bio-activity : | Measured by its binding ability in a functional ELISA. When Recombinant Human PDGFBB is coated at 1 μg/mL (100 μL/well), Biotinylated Recombinant Human PDGFRB Fc Chimera Avi-tag binds with an ED50 of 12-120 ng/mL. Measured by its ability to inhibit the biological activity of PDGF-BB using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 1-3 µg/mL. |
Molecular Mass : | 107-119 kDa, under reducing conditions |
Protein length : | Leu33-Lys531 |
Endotoxin : | <0.10 EU per 1 μg of the protein by the LAL method. |
Purity : | >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Applications : | Bioactivity |
Storage : | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution. |
Reconstitution : | Reconstitute at 500 μg/mL in PBS. |
Gene Name : | PDGFRB platelet-derived growth factor receptor, beta polypeptide [ Homo sapiens ] |
Official Symbol : | PDGFRB |
Synonyms : | PDGFRB; platelet-derived growth factor receptor, beta polypeptide; PDGFR; platelet-derived growth factor receptor beta; CD140b; JTK12; PDGFR1; PDGFR-beta; PDGF-R-beta; CD140 antigen-like family member B; platelet-derived growth factor receptor 1; beta-type platelet-derived growth factor receptor; CD140B; PDGFR-1; |
Gene ID : | 5159 |
mRNA Refseq : | NM_002609 |
Protein Refseq : | NP_002600 |
MIM : | 173410 |
UniProt ID : | P09619 |
Products Types
◆ Recombinant Protein | ||
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PDGFRB-2187H | Active Recombinant Human PDGFRB protein(Met1-Lys531), His-tagged | +Inquiry |
Pdgfrb-189M | Recombinant Mouse Pdgfrb Protein, His-tagged | +Inquiry |
PDGFRB-129H | Recombinant Human PDGFRB Protein (33-532aa), C-hIgG-His tagged | +Inquiry |
PDGFRB-33H | Active Recombinant Human PDGFRB Protein, His-tagged, Biotinylated | +Inquiry |
◆ Lysates | ||
PDGFRB-1107HCL | Recombinant Human PDGFRB cell lysate | +Inquiry |
PDGFRB-1112RCL | Recombinant Rat PDGFRB cell lysate | +Inquiry |
PDGFRB-1046CCL | Recombinant Cynomolgus PDGFRB cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1617 | U2OS PDGFRb Bioassay Kit | +Inquiry |
Kit-1618 | PDGFRb Functional Assay Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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