Recombinant Human PDHA1 Protein, MYC/DDK-tagged

• Cat.No.: PDHA1-825H
• Product Overview: Recombinant human PDHA1 protein, fused to MYC/DDK tag at C-terminus, was expressed in HEK293.

Specification

• Description: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010]
• Source: HEK293
• Species: Human
• Tag: MYC/DDK
• Form: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
• Molecular Mass: 40.2 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >50 ug/mL as determined by microplate BCA method

Gene Information

• Gene Name: pyruvate dehydrogenase E1 alpha 1 subunit[ Homo sapiens ]
• Official Symbol: PDHA1
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A
• Gene ID: 5160
• mRNA Refseq: NM_000284.3
• Protein Refseq: NP_000275
• MIM: 300502
• UniProt ID: P08559