Active Recombinant Human PGAM1 protein, His-tagged (Bioactivity Validated)
Cat.No. : | PGAM1-13H |
Product Overview : | Recombinant human PGAM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. |
- Specification
- Gene Information
- Related Products
Description : | PGAM1 belongs to the phosphoglycerate mutase family. This protein is important components of glucose and 2,3-BPGA (2,3-bisphosphoglycerate) metabolism and catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). Mutations in this protein cause muscle phosphoglycerate mutase efficiency, also known as glycogen storage disease X. |
Source : | E. coli |
Species : | Human |
Tag : | His |
Form : | Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 1mM DTT |
Bio-activity : | Specific activity is >300 units/mg, in which One unit will convert 1.0 umole of 3-phosphoglycerate to 2-phosphoglcerate per minute at pH 7.6 at 37centigrade. |
Molecular Mass : | 30.9 kDa (274aa), confirmed by MALDI-TOF |
AA Sequence : | MGSSHHHHHH SSGLVPRGSH MAAYKLVLIR HGESAWNLEN RFSGWYDADL SPAGHEEAKR GGQALRDAGY EFDICFTSVQ KRAIRTLWTV LDAIDQMWLP VVRTWRLNER HYGGLTGLNK AETAAKHGEA QVKIWRRSYD VPPPPMEPDH PFYSNISKDR RYADLTEDQL PSCESLKDTI ARALPFWNEE IVPQIKEGKR VLIAAHGNSL RGIVKHLEGL SEEAIMELNL PTGIPIVYEL DKNLKPIKPM QFLGDEETVR KAMEAVAAQG KAKK |
Purity : | > 90% by SDS - PAGE |
Storage : | Can be stored at +4centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20centigrade or -70centigrade. Avoid repeated freezing and thawing cycles. |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Gene Name : | PGAM1 phosphoglycerate mutase 1 (brain) [ Homo sapiens ] |
Official Symbol : | PGAM1 |
Synonyms : | PGAM1; PGAMA; phosphoglycerate mutase 1; PGAM B; nonmuscle form; BPG-dependent PGAM 1; PGAM-B; |
Gene ID : | 5223 |
mRNA Refseq : | NM_002629 |
Protein Refseq : | NP_002620 |
MIM : | 172250 |
UniProt ID : | P18669 |
Chromosome Location : | 10q25.3 |
Pathway : | Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycine, serine and threonine metabolism, organism-specific biosystem; |
Function : | bisphosphoglycerate 2-phosphatase activity; bisphosphoglycerate mutase activity; hydrolase activity; isomerase activity; phosphoglycerate mutase activity; phosphoglycerate mutase activity; protein kinase binding; |
Products Types
◆ Recombinant Protein | ||
PGAM1-0575H | Recombinant Human PGAM1 Protein (M1-K254), His/Strep tagged | +Inquiry |
Pgam1-4815M | Recombinant Mouse Pgam1 Protein, Myc/DDK-tagged | +Inquiry |
PGAM1-3204R | Recombinant Rhesus Macaque PGAM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PGAM1-4059R | Recombinant Rat PGAM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
PGAM1-0574H | Recombinant Human PGAM1 Protein (M1-K254), Tag Free | +Inquiry |
◆ Lysates | ||
PGAM1-3264HCL | Recombinant Human PGAM1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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