Recombinant Human PPP1R8 Protein (1-209 aa), GST-tagged

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Recombinant Human PPP1R8 Protein (1-209 aa), GST-tagged

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Cat.No. : PPP1R8-2143H
Product Overview : Recombinant Human PPP1R8 Protein (1-209 aa) is produced by E. coli expression system. This protein is fused with a GST tag at the N-terminal. Protein Description: Full Length of Isoform 2.
Description : Inhibitor subunit of the major nuclear protein phosphatase-1 (PP-1). It has RNA-binding activity but does not cleave RNA and may target PP-1 to RNA-associated substrates. May also be involved in pre-mRNA splicing. Binds DNA and might act as a transcriptional repressor. Seems to be required for cell proliferation. Isoform Gamma is a site-specific single-strand endoribonuclease that cleaves single strand RNA 3' to purines and pyrimidines in A+U-rich regions. It generates 5'-phosphate termini at the site of cleavage. This isoform does not inhibit PP-1. May be implicated in mRNA splicing.
Source : E. coli
Species : Human
Tag : GST
Form : Tris-based buffer,50% glycerol
Molecular Mass : 52.1 kDa
Protein length : 1-209 aa
AA Sequence : MGGEDDELKG LLGLPEEETE LDNLTEFNTA HNKRISTLTI EEGNLDIQRP KRKRKNSRVT FSEDDEIINP EDVDPSVGRF RNMVQTAVVP VKKKRVEGPG SLGLEESGSR RMQNFAFSGG LYGGLPPTHS EAGSQPHGIH GTALIGGLPM PYPNLAPDVD LTPVVPSAVN MNPAPNPAVY NPEAVNEPKK KKYAKEAWPG KKPTPSLLIL ENGTHMASSD ACHECKSMIA AAG
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : PPP1R8 protein phosphatase 1, regulatory subunit 8 [ Homo sapiens ]
Official Symbol : PPP1R8
Synonyms : PPP1R8; ard 1; ARD1; NIPP 1; NIPP1; PRO2047; RNase E; RD-1; NIPP-1;
Gene ID : 5511
mRNA Refseq : NM_002713
Protein Refseq : NP_002704
MIM : 602636
UniProt ID : Q12972

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