Recombinant Human PRMT9 Protein, FLAG-Strep-tagged

• Cat.No.: PRMT9-32H
• Product Overview: Recombinant human protein arginine methyltransferase 9 (NM_138364, a.a. 2-845) with an N-terminal FLAG-tag and a C-terminal Strep-tag was expressed in SF9 insect cell expression system.

Specification

• Description: This gene encodes a type II methyltransferase. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to the guanidino nitrogen atoms of arginine. The protein encoded by this gene methylates spliceosome associated protein 145 to regulate alternative splicing and acts as a modulator of small nuclear ribonucleoprotein maturation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
• Source: Insect Cells
• Species: Human
• Tag: FLAG-Strep
• Molecular Mass: 97 kDa
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Usage: 10 µl reaction mix containing assay buffer with Sadenosylmethionine, specific PRMT9 substrate, and PRMT9 (30-100 ng) added to the wells. Add antibody against methylated Arg residue and A-screen Acceptor beads. Incubate for 30 min, then add GSH conjugated Donor beads followed by Alpha Screening detection.
• Storage: At least 6 months at -80 centigrade. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80 centigrade.
• Storage Buffer: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml flag peptide.

Gene Information

• Gene Name: PRMT9 protein arginine methyltransferase 9 [ Homo sapiens (human) ]
• Official Symbol: PRMT9
• Synonyms: PRMT9; protein arginine methyltransferase 9; PRMT10; protein arginine N-methyltransferase 9; rotein arginine N-methyltransferase 10; protein arginine methyltransferase 10 (putative); putative protein arginine N-methyltransferase 10; putative protein arginine N-methyltransferase 9; EC 2.1.1.320
• Gene ID: 90826
• mRNA Refseq: NM_138364
• Protein Refseq: NP_612373
• MIM: 616125
• UniProt ID: Q6P2P2