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Recombinant Human PVR Protein, Mouse Fc-tagged

Cat.No. : PVR-622H
Product Overview : Recombinant Human PVR Protein (Trp 21 - Asn 343) (NP_006496.2), fused with a mouse IgG2a Fc tag at the C-terminus, was expressed in HEK293.
Source : HEK293
Species : Human
Tag : Mouse Fc
Predicted N Terminal : Trp 21
Form : Lyophilized from sterile 50 mM tris, 100 mM glycine, pH7.5, 10% trehalose.
Bio-activity : Measured by its binding ability in a functional ELISA. Immobilized Human TIGIT, Fc Tag at 2μg/mL (100 μL/well) can bind Human CD155, mouse IgG2a Fc tag with a linear range of 0.98-7.81 ng/mL.
Molecular Mass : Recombinant Human PVR Protein has a calculated MW of 62.2 kDa. As a result of glycosylation, The protein migrates as 70-100 kDa under reducing (R) condition and 130-160 kDa under non-reducing (NR) condition on SDS-PAGE gel.
Protein length : Trp 21 - Asn 343
Endotoxin : Less than 0.1 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : PVR poliovirus receptor [ Homo sapiens ]
Official Symbol : PVR
Synonyms : PVR; poliovirus receptor; PVS; CD155; HVED; Necl 5; NECL5; nectin like 5; Tage4; nectin-like 5; nectin-like protein 5; TAGE4; Necl-5; FLJ25946;
Gene ID : 5817
mRNA Refseq : NM_001135768
Protein Refseq : NP_001129240
MIM : 173850
UniProt ID : P15151
Chromosome Location : 19q13.2
Pathway : Adaptive Immune System, organism-specific biosystem; Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem;
Function : cell adhesion molecule binding; protein binding; receptor activity;

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