Description : |
Human PVRIG (poliovirus receptor related immunoglobulin domain-containing protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family. It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases. The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells. PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells. |
Source : |
HEK293 |
Species : |
Human |
Tag : |
Fc |
Predicted N Terminal : |
Thr41 & Glu43 |
Form : |
Lyophilized from a 0.2 μm filtered solution in PBS. See Certificate of Analysis for details. |
Bio-activity : |
Measured by its binding ability in a functional ELISA. When Recombinant Human PVRIG Fc Chimera is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human Nectin2/CD112 that produces 50% of the optimal binding response is 0.42.4 μg/mL. |
Molecular Mass : |
Predicted Molecular Mass: 40 kDa SDS-PAGE: 51-57 kDa, reducing conditions |
Endotoxin : |
<0.1 EU per 1 μg of the protein by the LAL method. |
Purity : |
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining. |
Storage : |
Store the unopened product at -20 to -70 centigrade. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution : |
Reconstitute at 500 μg/mL in PBS. |