Recombinant Human RNF34 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: RNF34-4156H
• Product Overview: RNF34 MS Standard C13 and N15-labeled recombinant protein (NP_079402) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. This protein also targets p53 and phospho-p53 for degradation. Alternatively splicing results in multiple transcript variants encoding distinct isoforms.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 41.6 kDa
• AA Sequence: MKAGATSMWASCCGLLNEVMGTGAV RGQQSAFAGATGPFRFTPNPEFSTY PPAATEGPNIVCKACGLSFSVFRKK HVCCDCKKDFCSVCSVLQENLRRCS TCHLLQETAFQRPQLMRLKVKDLRQ YLILRNIPIDTCREKEDLVDLVLCH HGLGSEDDMDTSSLNSSRSQTSSFF TRSFFSNYTAPSATMSSFQGELMDG DQTSRSGVPAQVQSEITSANTEDDD DDDDEDDDDEEENAEDRNPGLSKER VRASLSDLSSLDDVEGMSVRQLKEI LARNFVNYSGCCEKWELVEKVNRLY KENEENQKSYGERLQLQDEEDDSLC RICMDAVIDCVLLECGHMVTCTKCG KRMSECPICRQYVVRAVHVFKSTRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: RNF34 ring finger protein 34 [ Homo sapiens (human) ]
• Official Symbol: RNF34
• Synonyms: RNF34; ring finger protein 34, E3 ubiquitin protein ligase; ring finger protein 34; E3 ubiquitin-protein ligase RNF34; FLJ21786; RIF; RIFF; caspase regulator CARP1; RING finger protein RIFF; FYVE-RING finger protein MOMO; caspases-8 and -10-associated RING finger protein 1; human RING finger homologous to inhibitor of apoptosis protein; RFI; hRFI; CARP1; CARP-1
• Gene ID: 80196
• mRNA Refseq: NM_025126
• Protein Refseq: NP_079402
• MIM: 608299
• UniProt ID: Q969K3