Active Recombinant Human SAE1/UBA2, GST/His-tagged
Cat.No. : | SAE1-170H |
Product Overview : | Recombinant human SAE1 (amino acid residues 1-346), fused with N-terminal GST, Human UBA2 (amino acid residues 1-640), fused with N-terminal His, was expressed in E.coli. |
- Specification
- Gene Information
- Related Products
Description : | SAE1/SAE2 is a SUMO1, 2 and 3 E1 activating enzyme and functions as a heterodimer. Cloning of the human SAE1 and SAE2 genes was first described by Desterro et al. (1999). SAE1 and SAE2 share sequence similarity to the N-terminus and C-terminus of ubiquitin E1 activating enzymes respectively (Desterro et al. 1999). SAE2 harbours the E1-like active cysteine site while SUMO1 transfer to the E2 conjugating enzyme UBE2I requires both of the SAE subunits (Desterro et al. 1999). |
Source : | E. coli |
Species : | Human |
Tag : | GST/His |
Form : | 50 mM HEPES pH 7.5, 150 mM sodium chloride, 2 mM dithiothreitol, 10% glycerol |
Bio-activity : | E1 Thioester SUMO Loading Assay: The activity of GST-SAE1/His-SAE2 was validated by loading SUMO1 onto the active cysteine of GST-SAE1/His-SAE2. Incubation of the GST-SAE1/His-SAE2 enzyme in the presence of SUMO1 and ATP at 30?C was compared at two time points, T0 and T10 minutes. Sensitivity of the SUMO / GST-SAE1/His-SAE2 thioester bond to the reducing agent DTT was confirmed. |
Molecular Mass : | SAE1 = 64.8 kDa SAE2 = 73.4 kDa |
Purity : | >98% by SDS-PAGE |
Storage : | 12 months at -70°C. Avoid multiple freeze/thaw cycles. |
Concentration : | 0.5 mg/ml |
Gene Name : | SAE1 SUMO1 activating enzyme subunit 1 [ Homo sapiens ] |
Official Symbol : | SAE1 |
Synonyms : | SAE1; SUMO1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; activator Of sumo 1; AOS1; FLJ3091; Sua1; activator of SUMO1; SUMO-1 activating enzyme subunit 1; sentrin/SUMO-activating protein AOS1; SUMO-1 activating enzyme E1 N subunit; ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; SUA1; UBLE1A; HSPC140; |
Gene ID : | 10055 |
mRNA Refseq : | NM_001145713 |
Protein Refseq : | NP_001139185 |
MIM : | 613294 |
UniProt ID : | Q9UBE0 |
Chromosome Location : | 19q13.32 |
Pathway : | Adaptive Immune System, organism-specific biosystem; Antigen processing: Ubiquitination and Proteasome degradation, organism-specific biosystem; Class I MHC mediated antigen processing & presentation, organism-specific biosystem; Immune System, organism-specific biosystem; Ubiquitin mediated proteolysis, organism-specific biosystem; |
Function : | ATP-dependent protein binding; contributes_to SUMO activating enzyme activity; enzyme activator activity; ligase activity; nucleotide binding; protein C-terminus binding; protein binding; protein heterodimerization activity; protein heterodimerization activity; ubiquitin activating enzyme activity; |
Products Types
◆ Recombinant Protein | ||
SAE1-1949H | Recombinant Human SAE1 Protein, His (Fc)-Avi-tagged | +Inquiry |
SAE1-8623H | Recombinant Human SAE1 protein(Met1-Lys346), His&GST-tagged | +Inquiry |
SAE1-222H | Recombinant Human SAE1 Protein, MYC/DDK-tagged | +Inquiry |
Sae1-5678M | Recombinant Mouse Sae1 Protein, Myc/DDK-tagged | +Inquiry |
SAE1-4882R | Recombinant Rat SAE1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
SAE1-001HCL | Recombinant Human SAE1 cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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