Active Recombinant Human SEMA5B, Fc Chimera

• Cat.No.: SEMA5B-652H
• Product Overview: Recombinant Human SEMA5B (Accession # NP_001026872) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Human
• Tag: Fc Chimera
• Predicted N Terminal: Val78
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.When 5 x 10^4 cells/well are added to Recombinant Human Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 0.7 - 2.8 µg/mL.
• Molecular Mass: Recombinant Human SEMA5B, Fc Chimera has a calculated MW of 110.3 kDa (monomer). In SDS-PAGE migrates as 130-140 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: SEMA5B sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B [ Homo sapiens ]
• Official Symbol: SEMA5B
• Synonyms: SemG; SEMAG
• Gene ID: 54437
• mRNA Refseq: NM_001256348.1
• Protein Refseq: NP_001243277.1
• MIM: 609298
• UniProt ID: Q9P283
• Chromosome Location: 3q21.1