Recombinant Human SIGLEC10 protein, Mouse IgG2a Fc-tagged, low endotoxin

• Cat.No.: SIGLEC10-331H
• Product Overview: Recombinant Human SIGLEC10 protein (Met17-Thr546)(low endotoxin), fused to Mouse IgG2a Fc tag at C-terminus, was expressed in human 293 cells (HEK293).

Specification

• Description: The siglecs (sialic acid-binding Ig-like lectins) are a distinct subset of the Ig superfamily with adhesion-molecule-like structure. We describe here a novel member of the siglec protein family that shares a similar structure including five Ig-like domains, a transmembrane domain, and a cytoplasmic tail containing two ITIM-signaling motifs. Siglec-10 was identified through database mining of an asthmatic eosinophil EST library. The Siglec-10-VAP-1 interaction seems to mediate lymphocyte adhesion to endothelium and has the potential to modify the inflammatory microenvironment via the enzymatic end products.
• Source: HEK293
• Species: Human
• Tag: Mouse IgG2a Fc
• Form: Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 85.6 kDa. The protein migrates as 90-120 KDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 530
• Endotoxin: Less than 0.1 EU per ug by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: SIGLEC10
• Official Symbol: SIGLEC10
• Synonyms: SIGLEC10; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; MGC126774; PRO940; sialic acid binding Ig like lectin 10 Ig like lectin 7; SIGLEC 10; siglec like gene 2; SLG2; siglec-like gene 2; siglec-like protein 2; sialic acid binding Ig-like lectin 10 Ig-like lectin 7; SIGLEC-10
• Gene ID: 89790
• mRNA Refseq: NM_001171156
• Protein Refseq: NP_001164627
• MIM: 606091
• UniProt ID: Q96LC7