|Product Overview:||Recombinant Human SIRPA(Met 1-Arg 370) fused with His tag at C-terminal was expressed in HEK293.|
|Description:||The protein encoded by this gene is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are receptor-type transmembrane glycoproteins known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. This protein was found to participate in signal transduction mediated by various growth factor receptors. CD47 has been demonstrated to be a ligand for this receptor protein. This gene and its product share very high similarity with several other members of the SIRP family. These related genes are located in close proximity to each other on chromosome 20p13. Multiple alternatively spliced transcript variants have been determined for this gene.|
|Predicted N Terminal:||Glu 31|
|Form:||Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
|Bio-activity:||Measured by its binding ability in a functional ELISA.
Immobilized human SIRPA-His at 10 μg/ml (100 μl/well) can bind human CD47-Fc (Cat:12283-H02H). The EC50 of human CD47-Fc (Cat:12283-H02H) is 10.1-23.5 ng/ml.
|Molecular Mass:||The recombinant human SIRPA consists of 351 amino acids and predictes a molecular mass of 39 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSIRPA is approximately 45-50 kDa due to glycosylation.|
|Protein length:||Met 1-Arg 370|
|Endotoxin:||< 1.0 EU per μg of the protein as determined by the LAL method|
|Purity:||> 98 % as determined by SDS-PAGE|
|Stability:||Samples are stable for up to twelve months from date of receipt at -70 centigrade|
|Storage:||Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.|
|Reconstitution:||Reconstitute in PBS or others.|
|Shipping:||In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
|Gene Name:||SIRPA signal-regulatory protein alpha [ Homo sapiens ]|
|Synonyms:||SIRPA; signal-regulatory protein alpha; protein tyrosine phosphatase, non receptor type substrate 1 , PTPNS1; tyrosine-protein phosphatase non-receptor type substrate 1; BIT; CD172a; MFR; MYD 1; P84; SHPS 1; SHPS1; SIRP; SIRP ALPHA 1; SIRPalpha; SIRPalpha2; myd-1 antigen; inhibitory receptor SHPS-1; macrophage fusion receptor; CD172 antigen-like family member A; tyrosine phosphatase SHP substrate 1; brain-immunoglobulin-like molecule with tyrosine-based activation motifs; MYD-1; CD172A; PTPNS1;|