Recombinant Human SMYD2 Protein, His/SUMO-tagged SMYD2-019H

Recombinant Human SMYD2 Protein, His/SUMO-tagged

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Recombinant Human SMYD2 Protein, His/SUMO-tagged

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Cat.No. : SMYD2-019H
Product Overview : Recombinant Human SMYD2 protein (2-433) with a N-terminal His-SUMO-tag was expressed in E.coli
Description : SET domain-containing proteins, such as SMYD2, catalyze lysine methylation.
Source : E. coli
Species : Human
Tag : His/SUMO
Bio-activity : Activity was demonstrated using a radiometric assay with [3 H]-S-adenosylmethionine incubated with nucleosomes at room temperature overnight in 50 mM Tris-HCl, pH 8.0, containing 150 mM sodium chloride, 3 mM MgCl2, and 5% glycerol. Methylated products were separated by SDS-PAGE and visualized by exposure on autoradiographic film.
Molecular Mass : 62.7 kDa
Protein length : 2-433
Purity : ≥80% estimated by SDS-PAGE
Stability : ≥ 6 months
Storage : -80 centigrade
Storage Buffer : 50 mM Tris-HCl, pH 8.5, containing 400 mM sodium chloride, 1 mM DTT, and 20% glycerol
Shipping : Dry ice in continental US, may vary elsewhere.
SDS-PAGE Analysis of SMYD2 :

Autoradiograph of nucleosomes separated on SDS-PAGE following reaction with [3 H]-SAM and 2 µM SMYD2.

Lane 1: MW Markers
Lane 2: SMYD2 (4 µg)
Lane 3: SMYD2 (2 µg)

Gene Name : SMYD2 SET and MYND domain containing 2 [ Homo sapiens (human) ]
Official Symbol : SMYD2
Synonyms : SMYD2; SET and MYND domain containing 2; KMT3C; HSKM-B; ZMYND14; N-lysine methyltransferase SMYD2; SET and MYND domain-containing protein 2; histone methyltransferase SMYD2; lysine N-methyltransferase 3C; zinc finger, MYND domain containing 14; EC 2.1.1.354
Gene ID : 56950
mRNA Refseq : NM_020197
Protein Refseq : NP_064582
MIM : 610663
UniProt ID : Q9NRG4

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