Active Recombinant Human TEK Protein, hIgG/His-tagged

• Cat.No.: TEK-036H
• Product Overview: Recombinant human TEK protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
• Source: Insect cells
• Species: Human
• Tag: hIgG/His
• Form: Liquid
• Bio-activity: Measured by the ability of the binding activity in a functional ELISA. The ED50 range ≤1 μg/mL.
• Molecular Mass: 107.9 kDa
• AA Sequence: AMDLILINSLPLVSDAETSLTCIAS GWRPHEPITIGRDFEALMNQHQDPL EVTQDVTREWAKKVVWKREKASKIN GAYFCEGRVRGEAIRIRTMKMRQQA SFLPATLTMTVDKGDNVNISFKKVL IKEEDAVIYKNGSFIHSVPRHEVPD ILEVHLPHAQPQDAGVYSARYIGGN LFTSAFTRLIVRRCEAQKWGPECNH LCTACMNNGVCHEDTGECICPPGFM GRTCEKACELHTFGRTCKERCSGQE GCKSYVFCLPDPYGCSCATGWKGLQ CNEACHPGFYGPDCKLRCSCNNGEM CDRFQGCLCSPGWQGLQCEREGIPR MTPKIVDLPDHIEVNSGKFNPICKA SGWPLPTNEEMTLVKPDGTVLHPKD FNHTDHFSVAIFTIHRILPPDSGVW VCSVNTVAGMVEKPFNISVKVLPKP LNAPNVIDTGHNFAVINISSEPYFG DGPIKSKKLLYKPVNHYEAWQHIQV TNEIVTLNYLEPRTEYELCVQLVRR GEGGEGHPGPVRRFTTASIGLPPPR GLNLLPKSQTTLNLTWQPIFPSSED DFYVEVERRSVQKSDQQNIKVPGNL TSVLLNNLHPREQYVVRARVNTKAQ GEWSEDLTAWTLSDILPPQPENIKI SNITHSSAVISWTILDGYSISSITI RYKVQGKNEDQHVDVKIKNATITQY QLKGLEPETAYQVDIFAENNIGSSN PAFSHELVTLPESQAPADLGGGKML L
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Bioactivity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -115 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: TEK TEK receptor tyrosine kinase [ Homo sapiens (human) ]
• Official Symbol: TEK
• Synonyms: TEK; TEK receptor tyrosine kinase; TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B; angiopoietin-1 receptor; TEK tyrosine kinase, endothelial; endothelial tyrosine kinase; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2; EC 2.7.10.1
• Gene ID: 7010
• mRNA Refseq: NM_000459
• Protein Refseq: NP_000450
• MIM: 600221
• UniProt ID: Q02763