Active Recombinant Human TGFB1

• Cat.No.: TGFB1-428H
• Product Overview: TGFB1 Human Recombinant produced in Human 293 cells is a homodimeric polypeptide chain containing 2 x 112 amino acids and having a total molecular mass of 25kDa. The TGFB1 is purified by proprietary chromatographic techniques.

Specification

• Description: This gene encodes a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. Many cells have TGFB receptors, and the protein positively and negatively regulates many other growth factors. The secreted protein is cleaved into a latency-associated peptide (LAP) and a mature TGFB1 peptide, and is found in either a latent form composed of a TGFB1 homodimer, a LAP homodimer, and a latent TGFB1-binding protein, or in an active form composed of a TGFB1 homodimer. The mature peptide may also form heterodimers with other TGFB family members. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease.
• Source: Human 293 cells
• Species: Human
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. Lyophilized from a concentrated (1mg/ml) solution containing 10mM HCl & 50ug BSA per mg/ml of protein.
• Bio-activity: The ED50, as measured by the dose-dependent inhibition of IL-4-induced proliferation of mouse HT-2 cells is typically 0.1-0.15ng/ml.
• AA Sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS.
• Purity: Greater than 98.0% as determined by SDS-PAGE.
• Stability: Lyophilized TGFB1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TGFB1 Human should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Reconstitution: It is recommended to reconstitute the lyophilized TGFB1 in sterile 5mM HCl & 50ug/ml BSA at a concentration of 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Gene Information

• Gene Name: TGFB1 transforming growth factor, beta 1 [ Homo sapiens ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; CED; LAP; DPD1; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; Camurati-Engelmann disease; Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP
• Gene ID: 7040
• mRNA Refseq: NM_000660
• Protein Refseq: NP_000651
• MIM: 190180
• UniProt ID: P01137
• Chromosome Location: 19q13.1
• Pathway: ACE Inhibitor Pathway; Cardiac Progenitor Differentiation; Chagas disease (American trypanosomiasis)
• Function: antigen binding; enzyme binding; growth factor activity