|Product Overview:||Recombinant Human TNFRSF18 Protein, MIgG2a Fc-tagged, low endotoxin is expressed from human 293 cells (HEK293). It contains AA Gln 26 - Glu 161 (Accession # Q9Y5U5-1). Predicted N-terminus: Gln 26. This protein carries a mouse IgG2a Fc tag at the C-terminus.
The protein has a calculated MW of 41.4 kDa. The protein migrates as 46-49 kDa and 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
|Tag :||MIgG2a Fc|
|Form:||Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human GITR Ligand, Fc Tag at 5 μg/mL (100 μL/well) can bind Recombinant Human TNFRSF18 Protein, MIgG2a Fc-tagged, low endotoxin with a linear range of 0.039-0.313 μg/mL (QC tested).|
|Molecular Mass:||41.4 kDa|
|Protein length:||Gln 26 - Glu 161|
|Endotoxin:||Less than 0.1 EU per μg by the LAL method.|
|Purity:||>95% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||TNFRSF18 tumor necrosis factor receptor superfamily, member 18 [ Homo sapiens ]|
|Synonyms:||TNFRSF18; tumor necrosis factor receptor superfamily, member 18; AITR; CD357; GITR; GITR-D;|