Active Recombinant Human TNFRSF1B protein, His-GST-tagged
Cat.No. : | TNFRSF1B-446H |
Product Overview : | Recombinant Human TNFRSF1B protein(Ala298~Leu441), fused with His tag and GST tag, was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Human |
Tag : | His-GST |
Form : | 20mM Tris, 150mM NaCl, pH8.0, containing 0.05% sarcosyl and 5% trehalose. |
Bio-activity : | Tumor necrosis factor receptor superfamily member 1B (TNFRSF1B) , also known as tumor necrosis factor receptor 2 (TNFR2) and CD120b, is a membrane receptor that binds tumor necrosis factor-alpha (TNFα). This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. Besides, Tumor Necrosis Factor Alpha (TNFa) has been identified as an interactor of TNFRSF1B, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TNFRSF1B and recombinant human TNFa. Briefly, TNFRSF1B were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to TNFa-coated microtiter wells and incubated for 1h at 37 °C. Wells were washed with PBST and incubated for 1h with anti-TNFRSF1B pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 °C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TNFRSF1B and TNFa was shown in Figure 2, the EC50 was 0.0470~0.085 ug/ml. |
Molecular Mass : | 50kDa |
Protein Length : | Ala298~Leu441 |
Purity : | >95% |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 month. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. |
Gene Name : | TNFRSF1B tumor necrosis factor receptor superfamily, member 1B [ Homo sapiens ] |
Official Symbol : | TNFRSF1B |
Synonyms : | tumor necrosis factor receptor superfamily, member 1B; p75; TBPII; TNFBR; TNFR2; CD120b; TNFR1B; TNFR80; TNF-R75; p75TNFR; TNF-R-II; TNFRSF1B; tumor necrosis factor receptor 2; p75 TNF receptorL; soluble TNFR1B variant 1; tumor necrosis factor beta receptor; tumor necrosis factor binding protein 2 |
Gene ID : | 7133 |
mRNA Refseq : | NM_001066 |
Protein Refseq : | NP_001057 |
MIM : | 191191 |
UniProt ID : | P20333 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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