Recombinant Human TSLP (R127A, R130A) protein, His-tagged

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Recombinant Human TSLP (R127A, R130A) protein, His-tagged

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Cat.No. : TSLP-371H
Product Overview : Recombinant Human TSLP protein (R127A, R130A, Tyr29-Gln159), fused to His tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 16.7 kDa. The protein migrates as 22-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : 131
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of TSLP-371H :

Human TSLP (R127A, R130A), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA of TSLP-371H :

Immobilized Human TSLPR, Fc Tag at 5 μg/mL (100 μL/well) can bind Human TSLP (R127A, R130A), His Tag with a linear range of 0.8-6 ng/mL (QC tested).

Immobilized Human TSLP (R127A, R130A), His Tag at 1 μg/mL (100 μL/well) can bind Anti-TSLP, Human IgG2 with a linear range of 0.4-3 ng/mL (Routinely tested).

Bioactivity-SPR of TSLP-371H :

Captured Human TSLP (R127A, R130A), His Tag on CM5 Chip via anti-His antibody, can bind Human TSLP R, Fc Tag with an affinity constant of 4.22 nM as determined in SPR assay (Routinely tested).

Gene Name : TSLP
Official Symbol : TSLP
Synonyms : TSLP; thymic stromal lymphopoietin
Gene ID : 85480
mRNA Refseq : NM_033035
Protein Refseq : NP_149024
MIM : 607003
UniProt ID : Q969D9

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