Recombinant Human UBA3 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: UBA3-2793H
• Product Overview: UBA3 MS Standard C13 and N15-labeled recombinant protein (NP_937838) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 49.9 kDa
• AA Sequence: MADGEEPMAVDGGCGDTGDWEGRWN HVKKFLERSGPFTHPDFEPSTESLQ FLLDTCKVLVIGAGGLGCELLKNLA LSGFRQIHVIDMDTIDVSNLNRQFL FRPKDIGRPKAEVAAEFLNDRVPNC NVVPHFNKIQDFNDTFYRQFHIIVC GLDSIIARRWINGMLISLLNYEDGV LDPSSIVPLIDGGTEGFKGNARVIL PGMTACIECTLELYPPQVNFPMCTI ASMPRLPEHCIEYVRMLQWPKEQPF GEGVPLDGDDPEHIQWIFQKSLERA SQYNIRGVTYRLTQGVVKRIIPAVA STNAVIAAVCATEVFKIATSAYIPL NNYLVFNDVDGLYTYTFEAERKENC PACSQLPQNIQFSPSAKLQEVLDYL TNSASLQMKSPAITATLEGKNRTLY LQSVTSIEERTRPNLSKTLKELGLV DGQELAVADVTTPQTVLFKLHFTST RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: UBA3 ubiquitin-like modifier activating enzyme 3 [ Homo sapiens (human) ]
• Official Symbol: UBA3
• Synonyms: UBA3; ubiquitin-like modifier activating enzyme 3; UBE1C, ubiquitin activating enzyme E1C (homologous to yeast UBA3), ubiquitin activating enzyme E1C (UBA3 homolog, yeast); NEDD8-activating enzyme E1 catalytic subunit; hUba3; ubiquitin activating enzyme E1 homolog (yeast); NEDD8-activating enzyme E1C; Nedd8-activating enzyme hUba3; ubiquitin-activating enzyme 3; ubiquitin-like modifier-activating enzyme 3; UBA3, ubiquitin-activating enzyme E1 homolog; ubiquitin-activating enzyme E1C (UBA3 homolog, yeast); ubiquitin-activating enzyme E1C (homologous to yeast UBA3); UBE1C; hUBA3; MGC22384; DKFZp566J164
• Gene ID: 9039
• mRNA Refseq: NM_198195
• Protein Refseq: NP_937838
• MIM: 603172
• UniProt ID: Q8TBC4