| Description : |
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Myc/DDK |
| Molecular Mass : |
123.7 kDa |
| AA Sequence : |
MFSFEGDFKTRPKVSLGGASRKEEK ASLLHRTQEERRKREEERRRLKNAI IIQSFIRGYRDRKQQYSIQRSAFDR CATLSQSGGAFPIANGPNLTLLVRQ LLFFYKQNEDSKRLIWLYQNLIKHS SLFVKQLDGSERLTCLFQIKRLMSL CCRLLQNCNDDSLNVALPMRMLEVF SSENTYLPVLQDASYVVSVIEQILH YMIHNGYYRSLYLLINSKLPSSIEY SDLSRVPIAKILLENVLKPLHFTYN SCPEGARQQVFTAFTEEFLAAPFTD QIFHFIIPALADAQTVFPYEPFLNA LLLIESRCSRKSGGAPWLFYFVLTV GENYLGALSEEGLLVYLRVLQTFLS QLPVSPASASCHDSASDSEEESEEA DKPSSPEDGRLSVSYITEECLKKLD TKQQTNTLLNLVWRDSASEEVFTTM ASVCHTLMVQHRMMVPKVRLLYSLA FNARFLRHLWFLISSMSTRMITGSM VPLLQVISRGSPMSFEDSSRIIPLF YLFSSLFSHSLISIHDNEFFGDPIE VVGQRQSSMMPFTLEELIMLSRCLR DACLGIIKLAYPETKPEVREEYITA FQSIGVTTSSEMQQCIQMEQKRWIQ LFKVITNLVKMLKSRDTRRNFCPPN HWLSEQEDIKADKVTQLYVPASRHV WRFRRMGRIGPLQSTLDVGLESPPL SVSEERQLAVLTELPFVVPFEERVK IFQRLIYADKQEVQGDGPFLDGINV TIRRNYIYEDAYDKLSPENEPDLKK RIRVHLLNAHGLDEAGIDGGGIFRE FLNELLKSGFNPNQGFFKTTNEGLL YPNPAAQMLVGDSFARHYYFLGRML GKALYENMLVELPFAGFFLSKLLGT SADVDIHHLASLDPEVYKNLLFLKS YEDDVEELGLNFTVVNNDLGEAQVV ELKFGGKDIPVTSANRIAYIHLVAD YRLNRQIRQHCLAFRQGLANVVSLE WLRMFDQQEIQVLISGAQVPISLED LKSFTNYSGGYSADHPVIKVFWRVV EGFTDEEKRKLLKFVTSCSRPPLLG FKELYPAFCIHNGGSDLERLPTAST CMNLLKLPEFYDETLLRSKLLYAIE CAAGFELSTRTRPLEQKLISEEDLA ANDILDYKDDDDKV |
| Purity : |
> 80% as determined by SDS-PAGE and Coomassie blue staining |
| Stability : |
Stable for 3 months from receipt of products under proper storage and handling conditions. |
| Storage : |
Store at -80 centigrade. Avoid repeated freeze-thaw cycles. |
| Concentration : |
50 μg/mL as determined by BCA |
| Storage Buffer : |
100 mM glycine, 25 mM Tris-HCl, pH 7.3. |
