Recombinant Human YWHAZ Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: YWHAZ-226H
• Product Overview: YWHAZ MS Standard C13 and N15-labeled recombinant protein (NP_001129172) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 27.6 kDa
• AA Sequence: MDKNELVQKAKLAEQAERYDDMAAC MKSVTEQGAELSNEERNLLSVAYKN VVGARRSSWRVVSSIEQKTEGAEKK QQMAREYREKIETELRDICNDVLSL LEKFLIPNASQAESKVFYLKMKGDY YRYLAEVAAGDDKKGIVDQSQQAYQ EAFEISKKEMQPTHPIRLGLALNFS VFYYEILNSPEKACSLAKTAFDEAI AELDTLSEESYKDSTLIMQLLRDNL TLWTSDTQGDEAEAGEGGENTRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: YWHAZ tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide [ Homo sapiens (human) ]
• Official Symbol: YWHAZ
• Synonyms: YWHAZ; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3 monooxygenase/tryptophan 5 monooxygenase activation protein, delta polypeptide, YWHAD; 14-3-3 protein zeta/delta; 14 3 3 delta; 14 3 3 zeta; KCIP 1; 14-3-3 zeta; 14-3-3 delta; phospholipase A2; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; 14-3-3 protein/cytosolic phospholipase A2; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; YWHAD; KCIP-1; 14-3-3-zeta; MGC111427; MGC126532; MGC138156
• Gene ID: 7534
• mRNA Refseq: NM_001135700
• Protein Refseq: NP_001129172
• MIM: 601288
• UniProt ID: P63104