Active Recombinant Mouse Acpp Protein, His-tagged

• Cat.No.: Acpp-042M
• Product Overview: Recombinant mouse Prostatic Acid Phosphatase/ACPP protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: A non-specific tyrosine phosphatase that dephosphorylates a diverse number of substrates under acidic conditions (pH 4-6) including alkyl, aryl, and acyl orthophosphate monoesters and phosphorylated proteins. Has lipid phosphatase activity and inactivates lysophosphatidic acid in seminal plasma.
• Source: Insect cells
• Species: Mouse
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is >80,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37 centigrade.
• Molecular Mass: 41.3 kDa
• AA Sequence: KELKFVTLVFRHGDRGPIETFPTDP ITESSWPQGFGQLTQWGMEQHYELG SYIRKRYGRFLNDTYKHDQIYIRST DVDRTLMSAMTNLAALFPPEGISIW NPRLLWQPIPVHTVSLSEDRLLYLP FRDCPRFEELKSETLESEEFLKRLH PYKSFLDTLSSLSGFDDQDLFGIWS KVYDPLFCESVHNFTLPSWATEDAM IKLKELSELSLLSLYGIHKQKEKSR LQGGVLVNEILKNMKLATQPQKYKK LVMYSAHDTTVSGLQMALDVYNGVL PPYASCHMMELYHDKGGHFVEMYYR NETQNEPYPLTLPGCTHSCPLEKFA ELLDPVISQDWATECMATSSHQGRN
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -121 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Gene Information

• Gene Name: Acpp acid phosphatase, prostate [ Mus musculus (house mouse) ]
• Official Symbol: Acpp
• Synonyms: Acpp; acid phosphatase, prostate; Lap; PAP; Acp3; FRAP; Ppal; 5'-NT; A030005E02Rik; prostatic acid phosphatase; 5'-nucleotidase; TMPase; acid phosphatase 3; ecto-5'-nucleotidase; fluoride-resistant acid phosphatase; lysosomal acid phosphatase; thiamine monophosphatase; EC 3.1.3.2; EC 3.1.3.5
• Gene ID: 56318
• mRNA Refseq: NM_019807
• Protein Refseq: NP_062781
• UniProt ID: Q8CE08