Recombinant Mouse Atg4c Protein, Myc/DDK-tagged

• Cat.No.: Atg4c-1761M
• Product Overview: Purified recombinant protein of mouse full-length autophagy related 4C, cysteine peptidase (Atg4c), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 52.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Atg4c autophagy related 4C, cysteine peptidase [ Mus musculus (house mouse) ]
• Official Symbol: Atg4c
• Synonyms: ATG4C; autophagy-related 4C (yeast); cysteine protease ATG4C; autophagin 3; autophagin-3; AUT-like 3 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagy-related protein 4 homolog C; APG4 (ATG4) autophagy-related homolog C; cysteine protease involved in autophagy; autophagy-related cysteine endopeptidase 3; Apg4c; Autl1; Apg4-C; Atg4cl
• Gene ID: 242557
• mRNA Refseq: NM_001145967
• Protein Refseq: NP_001139439
• UniProt ID: Q811C2