Recombinant Mouse Carm1 Protein, Myc/DDK-tagged
Cat.No. : | Carm1-1966M |
Product Overview : | Purified recombinant protein of mouse full-length coactivator-associated arginine methyltransferase 1 (Carm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
- Specification
- Gene Information
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Description : | Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. |
Source : | HEK293T |
Species : | Mouse |
Tag : | Myc/DDK |
Molecular Mass : | 63.9 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | Carm1 coactivator-associated arginine methyltransferase 1 [ Mus musculus (house mouse) ] |
Official Symbol : | Carm1 |
Synonyms : | CARM1; coactivator-associated arginine methyltransferase 1; histone-arginine methyltransferase CARM1; protein arginine methyltransferase; protein arginine N-methyltransferase 4; Prmt4; MGC46828 |
Gene ID : | 59035 |
mRNA Refseq : | NM_153141 |
Protein Refseq : | NP_694781 |
UniProt ID : | Q9WVG6 |
Products Types
◆ Recombinant Protein | ||
CARM1-003H | Recombinant Human CARM1 Protein, GST-tagged | +Inquiry |
CARM1-797R | Recombinant Rat CARM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
CARM1-098H | Recombinant Human CARM1 Protein, GST-tagged | +Inquiry |
CARM1-503H | Recombinant Human CARM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
CARM1-1232M | Recombinant Mouse CARM1 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Lysates | ||
CARM1-7846HCL | Recombinant Human CARM1 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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