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Active Recombinant Mouse CD274 Antigen, Fc-tagged

Cat.No. : Cd274-1707M
Product Overview : The DNA sequence encoding the extracellular domain of mouse B7-H1 was fused to the carboxy-terminal Fc region of human IgG1 via a linker peptide, inserted into a suitable mammalian expression vector and expressed in NSO cells.
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  • Gene Information
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Description : B7-H1, also called programmed death ligand (PD-L1) is a protein in the B7 family of immune proteins. PD-L1 binds to its receptor, PD-1, found on activated T cells, B cells, and myeloid cells, to modulate activation or inhibition. Engagement of PD-L1 with PD-1 on T cells delivers a signal that inhibits TCR-mediated activation of IL-2 production and T cell proliferation. The mechanism involves inhibition of ZAP-70 phosphorylation and its association with CD247. PD-L1 signaling attenuates PKC-8 activation loop phosphorylation (resulting from TCR signaling), necessary for the activation of transcription factors NF-κB and AP-1 and for the production of IL-2. Upon IFN-γ stimulation, PD-L1 is expressed on T cells, NK cells, macrophages, myeloid DCs, B cells, epithelial cells, and vascular endothelial cells. Type I interferons can also upregulate PD-L1 on murine hepatocytes, monocytes, DCs, and tumor cells. It appears that upregulation of PD-L1 is a mechanism that cancers can employ to evade the host immune system as patients with higher expression levels of PD-L1 had a significantly poorer prognosis.
Source : Mammalian cells
Species : Mouse
Form : Lyophilized. This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Bio-activity : The biological activity of Mouse B7-H1 was determined by its ability to inhibit anti-CD3 induced proliferation of 72 hour PHA T cell blast. The expected ED50 for this effect is typically 2.5 - 10 μg/mL.
Molecular Mass : The predicted molecular weight of Recombinant Mouse B7-H1 is Mr 51.3 kDa (monomer). However, the actual molecular weight as observed by migration on SDS Page is Mr 75-85 kDa.
Endotoxin : <1.0 EU/µg as determined by the LAL method
Purity : >90% by SDS-PAGE and analyzed by silver stain.
Storage : This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Gene Name : Cd274 CD274 antigen [ Mus musculus (house mouse) ]
Official Symbol : Cd274
Synonyms : Cd274; B7h1; Pdl1; Pdcd1l1; Pdcd1lg1; A530045L16Rik; CD274 antigen; programmed cell death 1 ligand 1; B7 homolog 1; PDCD1 ligand 1; programmed death ligand 1
Gene ID : 60533
mRNA Refseq : NM_021893
Protein Refseq : NP_068693
UniProt ID : Q9EP73
Chromosome Location : 19; 19 C2
Pathway : Adaptive Immune System; Cell adhesion molecules (CAMs); Immune System
Function : protein binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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