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Active Recombinant Mouse CD47 protein, His-tagged

Cat.No. : CD47-382M
Product Overview : Recombinant Mouse CD47 protein(Q61735-1)(Gln19-Lys140) is expressed from HEK293 with His tag at the C-terminal.
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Bio-activity : Mouse SIRP alpha, His Tag immobilized on CM5 Chip can bind Mouse CD47, His Tag with an affinity constant of 1.33 μM as determined in SPR assay (Biacore T200).
Molecular Mass : The protein has a predicted MW of 14.9 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.
Protein Length : Gln19-Lys140
Endotoxin : Less than 1EU per μg by the LAL method.
Purity : > 95% as determined by Tris-Bis PAGE
> 95% as determined by HPLC
Storage : -20 to -80°C for 12 months as supplied from date of receipt.
-20 to -80°C for 3-6 months in unopened state after reconstitution.
2-8°C for 2-7 days after reconstitution.
Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
Reconstitution : Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Gene Name : Cd47 CD47 antigen (Rh-related antigen, integrin-associated signal transducer) [ Mus musculus ]
Official Symbol : CD47
Synonyms : CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Gene ID : 16423
mRNA Refseq : NM_010581
Protein Refseq : NP_034711
UniProt ID : Q61735

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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