Recombinant Mouse Chaf1a Protein, Myc/DDK-tagged

• Cat.No.: Chaf1a-2136M
• Product Overview: Purified recombinant protein of mouse full-length chromatin assembly factor 1, subunit A (p150) (Chaf1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Core component of the CAF-1 complex, a complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 101.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Chaf1a chromatin assembly factor 1, subunit A (p150) [ Mus musculus (house mouse) ]
• Official Symbol: Chaf1a
• Synonyms: CHAF1A; chromatin assembly factor 1, subunit A (p150); chromatin assembly factor 1 subunit A; CAF-Ip150; CAF-I p150; CAF-1 subunit A; CAF-I 150 kDa subunit; chromatin assembly factor I p150 subunit; CAF-1; Cac1p; AL023013; AL024058
• Gene ID: 27221
• mRNA Refseq: NM_013733
• Protein Refseq: NP_038761
• UniProt ID: Q9QWF0