Active Recombinant Mouse Ctla4 protein(Met1-Phe162), His-tagged
Cat.No. : | Ctla4-3307M |
Product Overview : | Recombinant Mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed in HEK293, with a polyhistidine tag at the C-terminus. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | C-His |
Protein length : | Met1-Phe162 |
Form : | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : | 1. Immobilized recombinant mouse CTLA4-His at 2 μg/ml (100 μl/well) can mouse CD80-Fc. The EC50 of mouse CD80-Fc is 1-9 ng/ml. 2. Immobilized recombinant mouse CTLA4-His at 10 μg/ml (100 μl/well) can human B7-1-Fc. The EC50 of human B7-1-Fc is 2-14 ng/ml. 3. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80. |
Molecular Mass : | The recombinant mouse CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15.46 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of mouse CTLA4 is approximately 25-30 kDa due to glycosylation. |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Gene Name : | Ctla4 cytotoxic T-lymphocyte-associated protein 4 [ Mus musculus ] |
Official Symbol : | Ctla4 |
Synonyms : | CTLA4; cytotoxic T-lymphocyte-associated protein 4; cytotoxic T-lymphocyte protein 4; CD152 antigen; cytotoxic T-lymphocyte-associated antigen 4; Cd152; Ly-56; Ctla-4; |
Gene ID : | 12477 |
mRNA Refseq : | NM_009843 |
Protein Refseq : | NP_033973 |
Products Types
◆ Recombinant Protein | ||
CTLA4-499H | Recombinant Human CTLA4 Protein (Met1-Phe162), HIgG1 Fc-tagged | +Inquiry |
CTLA4-4413B | Recombinant Bovine CTLA4 Protein | +Inquiry |
CTLA4-648C | Recombinant Cynomolgus monkey CTLA4 Protein, Fc-tagged | +Inquiry |
CTLA4-654M | Recombinant Mouse CTLA4 Protein, Fc-tagged | +Inquiry |
CTLA4-869H | Recombinant Human CTLA4 Protein, His&GST-tagged | +Inquiry |
◆ Lysates | ||
CTLA4-2179MCL | Recombinant Mouse CTLA4 cell lysate | +Inquiry |
CTLA4-2526HCL | Recombinant Human CTLA4 cell lysate | +Inquiry |
◆ Assay kits | ||
Kit-1713 | CTLA4[Biotinylated]:B7-2 Inhibitor Screening Assay Kit | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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