Recombinant Mouse Cyp7a1 Protein, Myc/DDK-tagged

• Cat.No.: Cyp7a1-2425M
• Product Overview: Purified recombinant protein of mouse full-length cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: A cytochrome P450 monooxygenase involved in the metabolism of endogenous cholesterol and its oxygenated derivatives (oxysterols). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Functions as a critical regulatory enzyme of bile acid biosynthesis and cholesterol homeostasis. Catalyzes the hydroxylation of carbon hydrogen bond at 7-alpha position of cholesterol, a rate-limiting step in cholesterol catabolism and bile acid biosynthesis (Probable). 7-alpha hydroxylates several oxysterols, including 4beta-hydroxycholesterol and 24-hydroxycholesterol. Catalyzes the oxidation of the 7,8 double bond of 7-dehydrocholesterol and lathosterol with direct and predominant formation of the 7-keto derivatives.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Cyp7a1 cytochrome P450, family 7, subfamily a, polypeptide 1 [ Mus musculus (house mouse) ]
• Official Symbol: Cyp7a1
• Synonyms: CYP7A1; cytochrome P450, family 7, subfamily a, polypeptide 1; cholesterol 7-alpha-monooxygenase; CYPVII; cytochrome P450 7A1; cytochrome P450, 7a1; cholesterol 7 alpha hydroxylase; cholesterol 7-alpha-hydroxylase
• Gene ID: 13122
• mRNA Refseq: NM_007824
• Protein Refseq: NP_031850
• UniProt ID: Q64505