Active Recombinant Mouse Eno2 protein, His-tagged

• Cat.No.: Eno2-125M
• Product Overview: Recombinant Mouse Eno2(1-434aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: Eno2, also known as Gamma enolase isoform1, is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release Eno2 into the blood. Eno2 is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 10% glycerol
• Bio-activity: Specific activity is > 10,000 pmol/min/ug, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
• Molecular Mass: 49.7kDa (457aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMSIEKIW AREILDSRGN PTVEVDLYTA KGLFRAAVPS GASTGIYEAL ELRDGDKQRY LGKGVLKAVD HINSRIAPAL ISSGISVVEQ EKLDNLMLEL DGTENKSKFG ANAILGVSLA VCKAGAAERD LPLYRHIAQL AGNSDLILPV PAFNVINGGS HAGNKLAMQE FMILPVGAES FRDAMRLGAE VYHTLKGVIK DKYGKDATNV GDEGGFAPNI LENSEALELV KEAIDKAGYT EKMVIGMDVA ASEFYRDGKY DLDFKSPADP SRYITGDQLG ALYQDFVRNY PVVSIEDPFD QDDWAAWSKF TANVGIQIVG DDLTVTNPKR IERAVEEKAC NCLLLKVNQI GSVTEAIQAC KLAQENGWGV MVSHRSGETE DTFIADLVVG LCTGQIKTGA PCRSERLAKY NQLMRIEEEL GDEARFAGHN FRNPSVL
• Purity: > 95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: Eno2 enolase 2, gamma neuronal [ Mus musculus ]
• Official Symbol: Eno2
• Synonyms: ENO2; enolase 2, gamma neuronal; gamma-enolase; neural enolase; neuron-specific enolase; 2-phospho-D-glycerate hydro-lyase; NSE; Eno-2; AI837106; D6Ertd375e
• Gene ID: 13807
• mRNA Refseq: NM_013509
• Protein Refseq: NP_038537
• UniProt ID: P17183
• Chromosome Location: 6 F2; 6 59.17 cM
• Pathway: Gluconeogenesis, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, organism-specific biosystem; Gluconeogenesis, oxaloacetate => fructose-6P, conserved biosystem; Glucose metabolism, organism-specific biosystem; Glycolysis, organism-specific biosystem
• Function: lyase activity; magnesium ion binding; metal ion binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity