| Source : |
HEK293 |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : |
1. Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA.2. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA.3. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA.4. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 2-10 ng/mL. |
| Molecular Mass : |
The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
| Protein Length : |
Met1-Arg192 |
| Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
| Purity : |
> 96 % as determined by SDS-PAGE |
| Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
