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Recombinant Mouse Fcgr1 Protein, His & Avi-tagged

Cat.No.: Fcgr1-838M
Product Overview: Recombinant extracellular domain (Met 1-Pro 297) of mouse FCGR1 (NP_034316.1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Source: HEK293
Species: Mouse
Tag: His & Avi
Predicted N Terminal: Glu 25
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse CD64-AVI at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC50 of biotinylated human IgG1 is 0.07-0.15 μg/ml.
Molecular Mass: The recombinant mouse FCGR1 consists of 308 amino acids after removal of the signal peptide and has a predicted molecular mass of 34.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FCGR1 is approximately 45-50 kDa due to glycosylation.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Fcgr1 Fc receptor, IgG, high affinity I [ Mus musculus ]
Official Symbol: Fcgr1
Synonyms: FCGR1; Fc receptor, IgG, high affinity I; high affinity immunoglobulin gamma Fc receptor I; fcRI; fc-gamma RI; igG Fc receptor I; IgG high affinity Fc receptor; CD64; IGGHAFC; AI323638; AV092959; FcgammaRI;
Gene ID: 14129
mRNA Refseq: NM_010186
Protein Refseq: NP_034316
MIM:
UniProt ID:
Pathway: Fc gamma R-mediated phagocytosis, organism-specific biosystem; Fc gamma R-mediated phagocytosis, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Leishmaniasis, organism-specifi
Function: IgG binding; IgG binding; IgG receptor activity; receptor activity;

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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