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Active Recombinant Mouse Fcgr1 Protein, His-tagged, Biotinylated

Cat.No. : Fcgr1-182M
Product Overview : Biotinylated Mouse Fc gamma RI / CD64 Protein (CD4-M52E8) is expressed from human HEK293 cells. It contains AA Glu 25 - Pro 297 (Accession # NP_034316).
  • Specification
  • Gene Information
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Source : HEK293
Species : Mouse
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
Bio-activity : The bioactivity is measured by its binding ability to mouse IgG2a in a SPR assay.Immobilized Biotinylated Mouse Fc gamma RI / CD64 Protein can bind mouse IgG2a with affinity constant of around 6.0 nM.Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Mouse Fc gamma RI with a linear range of 0.03-0.5 μg/mL.
Molecular Mass : This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 33 kDa. The predicted N-terminus is Glu 25. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation.
Protein length : Glu 25 - Pro 297
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.
Gene Name : Fcgr1 Fc receptor, IgG, high affinity I [ Mus musculus (house mouse) ]
Official Symbol : Fcgr1
Synonyms : Fcgr1; Fc receptor, IgG, high affinity I; high affinity immunoglobulin gamma Fc receptor I; IgG high affinity Fc receptor; fc-gamma RI; fcRI; igG Fc receptor I;
Gene ID : 14129
mRNA Refseq : NM_010186.5
Protein Refseq : NP_034316.1
UniProt ID : P26151

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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