Active Recombinant Mouse Fcgrt & B2m protein, His&Twin-Strep-tagged

• Cat.No.: Fcgrt & B2m-1545M
• Product Overview: Recombinant Mouse Fcgrt & B2m protein(Ser 22 - Ser 297 (FCGRT) & Ile 21 - Met 119 (B2M)), fused with His and Twin-Strep tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His&Twin-Strep
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Ipilumimab at 5 μg/mL (100 μL/well) can bind Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag with a linear range of 0.625-5 μg/mL.
• Molecular Mass: Mouse FCGRT&B2M Heterodimer Protein, His Tag&Twin-Strep Tag, produced by co-expression of FCGRT and B2M, has a calculated MW of 32.9 kDa (FCGRT) and 14.7 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Twin-Strep tag at the C-terminus. The reducing (R) protein migrates as 42-48 kDa (FCGRT) and 15-17 kDa (B2M) respectively due to glycosylation.
• Protein Length: Ser22-Ser297(FCGRT)&Ile21-Met119(B2M)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please protect from light and avoid repeated freeze-thaw cycles.This product is stable after at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgrt Fc receptor, IgG, alpha chain transporter [ Mus musculus ]
• Official Symbol: FCGRT
• Synonyms: FCGRT; Fc receptor, IgG, alpha chain transporter; IgG receptor FcRn large subunit p51; neonatal Fc receptor; igG Fc fragment receptor transporter alpha chain; FcRn
• Gene ID: 14132
• mRNA Refseq: NM_010189
• Protein Refseq: NP_034319