Active Recombinant Mouse Fgf7

• Cat.No.: Fgf7-597M
• Product Overview: Recombinant Mouse Fgf7 (Accession # NP_032034) Cys32-Thr194, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Predicted N Terminal: Cys32
• Form: Lyophilized from a 0.2 µm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using 4MBr-5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802.The ED50 for this effect is typically 10 - 50 ng/mL.
• Molecular Mass: Recombinant Mouse Fgf7 has a calculated MW of 18.7 kDa. In SDS-PAGE migrates as 19 kDa, reducing conditions.
• Protein length: Cys32-Thr194
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Epyc epiphycan [ Mus musculus (house mouse) ]
• Official Symbol: Epyc
• Synonyms: Dspg3; PG-Lb; SLRR3B; epiphycan; epiphycan; dermatan sulfate proteoglycan 3; dermatan sulphate proteoglycan 3; proteoglycan-Lb; small chondroitin/dermatan sulfate proteoglycan
• Gene ID: 13516
• mRNA Refseq: NM_007884.2
• Protein Refseq: NP_031910.1
• UniProt ID: P70186
• Chromosome Location: 10 50.35 cM; 10 C2-C3