Recombinant Mouse Foxo3 Protein, Myc/DDK-tagged

• Cat.No.: Foxo3-3078M
• Product Overview: Purified recombinant protein of mouse full-length forkhead box O3 (Foxo3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Transcriptional activator that recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3' and regulates different processes, such as apoptosis and autophagy. Acts as a positive regulator of autophagy in skeletal muscle: in starved cells, enters the nucleus following dephosphorylation and binds the promoters of autophagy genes, such as GABARAP1L, MAP1LC3B and ATG12, thereby activating their expression, resulting in proteolysis of skeletal muscle proteins. Triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34c expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation. In response to metabolic stress, translocates into the mitochondria where it promotes mtDNA transcription. Also acts as a key regulator of chondrogenic commitment of skeletal progenitor cells in response to lipid availability: when lipids levels are low, translocates to the nucleus and promotes expression of SOX9, which induces chondrogenic commitment and suppresses fatty acid oxidation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 71.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Foxo3 forkhead box O3 [ Mus musculus (house mouse) ]
• Official Symbol: Foxo3
• Synonyms: FOXO3; forkhead box O3; forkhead box protein O3; forkhead box O3a; forkhead protein FKHR2; Fkhr2; C76856; FKHRL1; Foxo3a; 1110048B16Rik; 2010203A17Rik
• Gene ID: 56484
• mRNA Refseq: NM_019740
• Protein Refseq: NP_062714
• UniProt ID: Q9WVH4