Recombinant Mouse G3bp1 Protein, Myc/DDK-tagged

• Cat.No.: G3bp1-3122M
• Product Overview: Purified recombinant protein of mouse full-length GTPase activating protein (SH3 domain) binding protein 1 (G3bp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 51.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: G3bp1 GTPase activating protein (SH3 domain) binding protein 1 [ Mus musculus (house mouse) ]
• Official Symbol: G3bp1
• Synonyms: G3bp1; GTPase activating protein (SH3 domain) binding protein 1; G3bp; C87777; AI849976; mKIAA4115; B430204O07; ras GTPase-activating protein-binding protein 1; ATP-dependent DNA helicase VIII; G3BP-1; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; HDH-VIII; Ras-GTPase-activating protein SH3-domain binding protein 1; EC 3.6.4.12; EC 3.6.4.13
• Gene ID: 27041
• mRNA Refseq: NM_013716
• Protein Refseq: NP_038744
• UniProt ID: P97855