Recombinant Mouse Gapdh Protein, Myc/DDK-tagged

• Cat.No.: Gapdh-3150M
• Product Overview: Purified recombinant protein of mouse full-length glyceraldehyde-3-phosphate dehydrogenase (Gapdh), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Alternative splicing results in multiple transcript variants. Many pseudogenes similar to this locus are found throughout the mouse genome.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 35.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gapdh glyceraldehyde-3-phosphate dehydrogenase [ Mus musculus (house mouse) ]
• Official Symbol: Gapdh
• Synonyms: Gapdh; glyceraldehyde-3-phosphate dehydrogenase; Ga; Gapd; glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; EC 1.2.1.12
• Gene ID: 14433
• mRNA Refseq: NM_008084
• Protein Refseq: NP_032110
• UniProt ID: P16858