Recombinant Mouse Gtf2e1 Protein, Myc/DDK-tagged

• Cat.No.: Gtf2e1-3324M
• Product Overview: Purified recombinant protein of mouse full-length general transcription factor II E, polypeptide 1 (alpha subunit) (Gtf2e1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 49.6 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Gtf2e1 general transcription factor II E, polypeptide 1 (alpha subunit) [ Mus musculus (house mouse) ]
• Official Symbol: Gtf2e1
• Synonyms: Gtf2e1; general transcription factor II E, polypeptide 1 (alpha subunit); FE; 56kD; TFII; 56kDa; TFIIE-A; AI303800; 2610024P03Rik; general transcription factor IIE subunit 1; TFIIE-alpha; general transcription factor II E, polypeptide 1 (alpha subunit, 56kDa); general transcription factor IIE 56 kDa subunit; general transcription factor IIE, polypeptide 1 (alpha subunit, 56kDa); transcription initiation factor IIE subunit alpha
• Gene ID: 74197
• mRNA Refseq: NM_028812
• Protein Refseq: NP_083088
• UniProt ID: Q9D0D5