Active Recombinant Mouse Il17a Protein, His-Tagged
Cat.No. : | Il17a-01M |
Product Overview : | Recombinant mouse Il17a Protein, His-tagged was expressed in E. coli cell |
- Specification
- Gene Information
- Related Products
Description : | Interleukin-17A is a protein that in humans is encoded by the IL17A gene. The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). |
Source : | Escherichia coli |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized powder |
AA Sequence : | MAAIIPQSSACPNTEAKDFLQNVKV NLKVFNSLGAKVSSRRPSDYLNRST SPWTLHRNEDPDRYPSVIWEAQCRH QRCVNAEGKLDHHM NSVLIQQEILVLKREPESCPFTFRV EKMLVGVGCTCVASIVRQAA with polyhistidine tag at the C-terminus |
Endotoxin : | <0.1 EU per 1 μg of the protein by the LAL method. |
Bio-activity : | Measure by its ability to induce IL-6 secretion in 3T3 cells. The ED50 for this effect is <1 ng/mL. The specific activity of recombinant mouse IL-17A is > 1 x 10^6 IU/mg. |
Purity : | >98% as determined by SDS-PAGE. Ni-NTA chromatography |
Storage Buffer : | The protein was lyophilized from a solution containing 20 mM sodium citrate, 0.2 M NaCl, pH 4.5. |
Reconstitution : | It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved. |
Storage : | Lyophilized protein should be stored at -20°C. Upon reconstitution, protein aliquots should be stored at -20°C or -80°C. |
Notes : | Please use within one month after protein reconstitution. |
Publication : |
Oscilatory PEEP (BiPEEP) Improves Oxygenation and Reduces Inflammation in an Experimental Model of ARDS (2023)
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Gene Name : | Il17a interleukin 17A [ Mus musculus (house mouse) ] |
Official Symbol : | Il17a |
Synonyms : | Il17; Ctla8; IL-17; Ctla-8; IL-17A |
Gene ID : | 16171 |
mRNA Refseq : | NM_010552.3 |
Protein Refseq : | NP_034682.1 |
UniProt ID : | Q60971 |
Products Types
◆ Recombinant Protein | ||
IL17A-254I | Active Recombinant Human IL17A Protein (142 aa), His-tagged | +Inquiry |
IL17A-48H | Recombinant Human IL17A Protein | +Inquiry |
Il17a-52M | Active Recombinant Mouse Il17a Protein (Ala26-Ala158), C-His tagged, Animal-free, Carrier-free | +Inquiry |
Il17a-094M | Active Recombinant Mouse Il17a Protein | +Inquiry |
IL17A-142R | Recombinant Rat IL-17AF Heterodimer Protein | +Inquiry |
◆ Lysates | ||
IL17A-001HCL | Recombinant Human IL17A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
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Q&As (10)
Ask a questionAnimal models, such as experimental autoimmune encephalomyelitis, explore IL17A's role in autoimmunity.
Inducing IL17A expression in T cells involves culturing them with specific cytokines.
Studying IL17A's influence on tissue-specific inflammation in humans involves analyzing patient samples.
Neutralizing antibodies block IL17A's effects in cell cultures or animal models.
Multiplex cytokine assays use fluorescent beads to measure IL17A and other cytokines simultaneously.
IL17RA signaling is triggered by IL17A binding and is studied through downstream phosphorylation assays.
Methods like transepithelial electrical resistance (TEER) assay evaluate IL17A's effect on barrier function.
Inflammatory models, like airway inflammation assays, examine IL17A's impact on neutrophil recruitment.
Flow cytometry or intracellular staining help identify IL17A-producing cell subsets within tissues.
Experimental designs combine cancer cell lines with IL17A stimulation to investigate its pro-tumor effects.
Customer Reviews (3)
Write a reviewGood for receptor studies.
Effective in Th17 differentiation.
High bioactivity in various tissue types.
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